Strategies for Postprocessing Non-Natural Modifications of Commercially Produced Magnetosomes

Author(s):

Alexander Winton, Mark A. Allen, and Matthew Coppock

Abstract:

Magnetosomes are nanoscale magnetic particles encased in a phospholipid bilayer synthesized in magnetotactic bacteria. In this work, magnetosomes were characterized and chemically modified to explore their potential for bio-integration and material science applications. Fourier-transform infrared spectroscopy confirmed the presence of a phospholipid membrane surrounding the iron-oxide core of magnetosomes, differentiating them from commercially sourced magnetite. Initial attempts at aqueous dispersion proved challenging, with solvent viscosity positively correlating to suspension stability. Subsequent chemical modifications—utilizing fluorescein isothiocyanate, carbodiimide chemistry, and cholesterol anchoring—demonstrated successful surface functionalization. Biotinylation and cellulose binding peptide modification were also achieved, leading to the creation of magnetically responsive cotton textiles with approximately 5.5% (w/w) magnetite loading. These results provide a foundation for future genetic engineering and chemical modification strategies aimed at enhancing magnetosome functionality and biocompatibility.